Gatewaytm bp clonasetm
WebThe Donor Vector box, the BP Clonase Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1-4) contain the following items. Store the BP Clonase ™ enzyme mix at -80°C. Store all other components at -20°C. Store Zeocin ™ at -20°C, protected from light. Note: http://csrri.iit.edu/~howard/biol446/Lab1.bp_clonaseii_man.pdf
Gatewaytm bp clonasetm
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WebPart Name: attB4: Description: BP Clonase TM, core recombination site for the Gateway(R) BP reaction, encoded by mutant version of attB: Source: GenSmart Design Collection: Publication: Size (bp) 21 http://csrri.iit.edu/~howard/biol446/Lab1.bp_clonaseii_man.pdf
WebGateway® BP Clonase II Enzyme Mix Cat. No. 11789-020 Size: 20 reactions Cat. No. 11789-100 Size: 100 reactions Store at -20°C (non-frost-free freezer) Gateway® Technology The Gateway® Technology is a universal cloning method that takes advantage of the site-specific recombination properties of bacteriophage lambda (1) to provide WebJan 1, 2013 · Gateway Clonase Enzymes (back to Table of Contents) (back to Components) Name Size Catalog Number. Gateway BP Clonase Enzyme Mix 20 rxns 11789013. 100 rxns 11789021. BP clonase II enzyme mix (pre-mixed ready-to-use 20 rxns 11789020. solution of clonase and reaction buffer) 100 rxns 11789100. Gateway LR …
WebEvent-based gateway. Parallel gateway. Parallel event-based gateway. Inclusive gateway. Complex gateway. BPMN gateways are decision points that can adjust the path of a flow … WebCatalog number: 11789100. Gateway™ BP Clonase™ II enzyme mix catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing att …
WebDec 6, 2014 · BP clonase: 2 ul, 25 o C incubation for more than 24 hours. reaction was stopped by adding pronease k and incubation for 10 min at 37 o C. Cells used for …
WebBP Clonase® II Enzyme Mix The BP Clonase® II enzyme mix (Box 2) contains the followin g rea ents. Store Box 2 at –20°C for up to 6 months. For long-term storage, store at –80°C. Item Composition Quantity BP Clonase® II Enzyme Mix Proprietary 40 μl Proteinase K solution 2 μg/μl in: 10 mM Tris-HCl, pH 7.5 20 mM CaCl 2 50% glycerol 40 μl 1l柴油发多少电WebFeb 11, 2024 · by a BP reaction (GatewayTM BP ClonaseTM II Enzymemix, Invitrogen) to generate pENTR-BORIS with six C-terminal His-tags followed by a stop codon. To generate pI20-BORIS-6xH the entry clone was recombined into the lentiviral destination vector pI20 (empty vector (EV)-6xH, a kind gift from 1l柴油发电WebGateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction takes … 1l有多重Web8. Remove the 5 x BP-Clonase TM II enzyme mix from -20°C. It is most efficiently mixed by pipetting up and down, do not vortex. 9. Pipett 1 µl of this BP-Clonase TM II enzyme mix to the Gateway ® reaction. This is expensive stuff don’t leave it to rot in the ice-bucket! 10. Store the enzyme mix and buffer immediately at -20°C !!! 1l柴油多少钱WebAll Gateway™ BP Clonase™ enzyme kits include proteinase K solution (2 µg/µl), 30% PEG 8000/30 mM MgCl2 Solution and a positive control vector. Store BP Clonase™ enzyme at -80°C; BP Clonase™ II Enzyme mixtures at -20°C. Guaranteed stable for 6 months when properly stored. Enzyme. BP Clonase. 1l柴油多重WebHere are the Basics is Gateway® Reaction including BP respond, LR reaction, One Tube standard and Gateway Vector Conversion 1l有多少立方米WebBP反应是利用一个attB DNA片段或表达克隆和一个attP 供体载体之间的重组反应，创建一个入门克隆。 ... （2）混合包含目的基因的入门克隆和合适的目的载体以及Gateway LR Clonase酶，构建表达克隆。（表达克隆用来在合适的宿主中进行蛋白 的表达和分析。 ... 1l柴油排放多少二氧化碳