Hbad-mcherry-egfp-lc3
WebmCherry, tdTomato, Red Fluorescent Protein (dsRed) Cross Reactivity. This antibody does not cross-react with green fluorescent protein. Immunogen. Recombinant mCherry … WebNov 19, 2024 · HBAD-mcherry-EGFP-LC3 adenovirus (MOI = 50) was used to infect rat articular chondrocytes for 24 h according to the manufacturer’s instructions (Hanbio, Shanghai, China).
Hbad-mcherry-egfp-lc3
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WebPremo Autophagy sensors take advantage of autophagy-associated proteins LC3B or p62, along with GFP or RFP, to detect either the induction or inhibition of autophagy in cells. The Premo Autophagy sensors are … Web,南模生物是国内模式生物领域领先的生物科技公司，专业从事遗传修饰动物模型(转基因小鼠、斑马鱼、线虫)的研发、饲养 ...
WebThe mouse was infected with mCherry-EGFP-LC3 adeno-associated virus (100 μL) via the tail vein four weeks before sacrifice. The liver was removed carefully and trimmed immediately after the mouse was sacrificed. Then, the tissue was embedded in optimal cutting temperature (OCT) compound (Sakura, Torrance, USA), serial sections (6 μm) … WebAug 24, 2024 · First, a previously cloned pLenti-X1-pEF-mCherry-eGFP-LC3 construct (by LR clonase reaction) was cut using BamHI and XbaI to liberate the insert. All genes of interest were then Gibson assembled using Gibson Assembly Master Mix (E2611; NEB) together with the desired epitope or fluorescence tag into the pLenti-X1-DEST vector …
WebNov 19, 2024 · HBAD-mcherry-EGFP-LC3 adenovirus (MOI = 50) was used to infect rat articular chondrocytes for 24 h according to the manufacturer’s instructions (Hanbio, Shanghai, China). WebDownload. mcherry-GFP-Lc3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow cytometry using reporter ...
Web(2) tfLC3 mice express RFP-EGFP-LC3 or mCherry-EGFP-LC3 for measuring autophagy flux. The num-bers of GFP CRFP puncta (yellow) and RFP puncta (red) are counted. Yellow puncta represent phagophores and autophagosomes, and red puncta represent autolyso-somes. (3) GFP-LC3-RFP-LC3DG mice express GFP-LC3-RFP-LC3DG for …
ps9151-f3-axA detailed list of materials is provided in Table 6. Important: All hits in screening approaches have to be verified using orthogonal methods to assess autophagy (for a comprehensive … See more A detailed list of materials is provided in Table 5. 1. 2.1. Grow cells in either F-bottom (adherent cell types) or V-bottom (suspension cell types) 96-well plates. Seeding of 50,000 cells per well for adherent cells and … See more For the construction of the cell lines use low passage numbers. For accurate autophagy measurements, make sure that the cells never overgrew, even during the construction of the … See more retro americn flagmotorcycle helmetsWebMCF7 cells stably expressing the fusion protein enhanced green fluorescent protein (eGFP)–LC3 (MCF7 eGFP-LC3 cells) were used to investigate the LC3 response upon ablation laser–induced plasma membrane injury. ps8 playstationWebF1: Figure 1. mCherry-GFP-LC3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow … ps 8 shirlee solomonWebTandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. … retro american football sweatshirtsWebLC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in … ps 8 power supplyWebGenome-wide association studies (GWAS) have identified hundreds of genetic risk regions for kidney dysfunction [estimated glomerular filtration rate (eGFR)]; however, the … retro and vintage wholesale