Phospho-akt1/2/3 ser473 cellular kit
WebSep 12, 2015 · Inhibition CellularAkt semisyntheticviridins epidermalgrowth factor–dependent activation HT-29colon cancer cells Westernhybrid- ization using anti-phospho-Ser 473 -Akt antibody (Fig. 2A). Quantitation phospho-Ser473 totalAkt (Fig. 2B) gave IC 50 values (FSE) 2.2nmol/L, PX-867 3.5nmol/L, WebThe phospho-EGFR assay kit is designed for the cell-based quantitative detection of Tyr1068 phosphorylation on EGFR. Mutations that lead to an overexpression or hyperactivity of EGFR, also known as ErbB1 or HER1, are associated with a number of cancers, such as lung, colorectal, prostate, glioblastoma cancer, etc.
Phospho-akt1/2/3 ser473 cellular kit
Did you know?
WebPhospho-AKT1/2/3 (Ser473) cellular kit HTRF® Kit Diabetes and metabolic disorders A portfolio to tackle in-depth diabetes and metabolic disorders studies Explore Phospho-AKT1/2/3 (Ser473) control lysate HTRF® Control lysate Phospho-AKT1/2/3 (Thr308) cellular kit HTRF® Kit Phospho-AKT1/2/3 (Thr308) control lysate HTRF® Control lysate Guides WebNov 25, 2015 · The ER also plays a critical role toward maintenance of cellular calcium homeostasis. ... and the following oligonucleotide primers were used for RT-PCR: Akt1 forward, 5′-CTGTCTCGAGAGCGTGTGTT-3′; Akt1 reverse, 5′-AACAGCTTCTCGTGGTCCTG-3′; Akt2 ... [rabbit polyclonal anti-phospho-Akt (Ser473) antibody (Cell Signaling Technology, …
WebMyricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β WebThe FastScan™ Phospho-Akt (Ser473) ELISA Kit detects endogenous levels of Akt when phosphorylated at Ser473 as shown in Figure 1. This kit detects proteins from the …
WebIn the case of AKT1/2, immunofluorescence is reduced following the augmentation of NP-427 concentrations (Figure 8A–D). Similar to the anterior cases, the Western blot showed a decrease in phospho-Thr308-Akt, phospho-Ser473-Akt, and total AKT at 24 h in cells treated with NP-427 at 0.5 and 0.75 mg/mL ( p < 0.05) ( Figure 8 E). WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin …
WebSep 15, 2024 · The protein concentration of the supernatants was measured by BCS TM protein assay kit according to manufacturer’s ... The knockout of STK24 expression did …
Web#IR172-668 / IR172-668,Phospho-AKT (Ser473) antibody. Products ... and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. ... Subsequent phosphorylation of both threonine residue 308 and serine ... earthtrack group perthWebDec 16, 2009 · PIP3 recruits protein dependent kinase 1 (PDK1) and AKT, also known as protein kinase B (PKB), to the plasma membrane where AKT is phosphorylated at Thr308 by PDK1 and at Ser473 by mTOR complex 2 (TORC2) . AKT1 can also be phosphorylated at Ser473 by DNA-PK in the nucleus . AKT occupies a key regulatory node in the PI3K … earth trackers portlandWebBath Filler and Heat Transfer Fluids; Bath Specific Platforms and Clamps; Bath Specific Tube Racks; Calibration Baths; Circulating Baths; Coliform Baths ctrl alt del church of gaming final pageWebThis HTRF kit is designed to monitor AKT1 phosphorylation on Ser473 as a readout of Pi3K pathway ... Learn More Phospho-AKT1/2/3 (Ser473) cellular kit ctrl alt c windows 10WebPhospho-AKT1/2/3 (Ser473) control lysate HTRF®. Phospho-AKT1/2/3 (Ser473) control lysate. This lysate is a component of the phospho-AKT1/2/3 (Ser473) kit. It may be used … ctrl alt c windowsWeb(Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. Please visit www.cellsignal.com for a complete listing of recommended companion products. #7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit Species Cross-Reactivity: H, M, R … ctrlaltdel console war tvtropesWebphospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent Positive Control Cell Lysate (lyophilized) Detection Reagent (TMB Substrate) Stop Solution Plate seal Description … earthtrack rail and civil