Polyhydroxybutyrate depolymerase
WebNov 22, 2000 · The linear and cyclic oligomers containing from 2 to 10 repeating units were hydrolyzed in solutions of the depolymerase isolated from Aspergillus fumigatus and Alcaligenes faecalis, ... Single Crystal Revealed by Use of a Mutant of Polyhydroxybutyrate Depolymerase. Biomacromolecules 2002, 3 (2) , 312-317. WebTwo methods for accurate poly (3-hydroxybutyrate) (PHB) depolymerase activity determination and quantitative and qualitative hydrolysis product determination are …
Polyhydroxybutyrate depolymerase
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Web1 INTRODUCTION. The study of extracellular polyhydroxybutyrate (PHB) depolymerases (PhaZs) produced by a variety of microorganisms (Jendrossek, 2005; Knoll et al., 2009; … WebPolyhydroxyalkanoates (PHA) are biodegradable polymers that are attractive materials for use in tissue engineering and medical device manufacturing. Ralstonia eutropha is regarded as the model organism for PHA biosynthesis. We examined the effects of
WebSep 7, 2024 · Strain DSDY0501 with polyhydroxybutyrate (PHB)-degrading ability was isolated from activated sludge. The morphological, physiological, and biochemical … A total of 79 bacterial strains were successfully isolated from the wastewater treatment area of Kasetsart University, Bangkok, Thailand. Nile red agar plates were used for preliminary screening to select PHB-producing strains. Ten strains exhibited bright orange fluorescence under UV light after being incubated … See more To identify BSRT1-1, the 16S rRNA gene of strain BSRT1-1 was extracted and sequenced. The sequence of the 16S rRNA gene (1,483 bp) was obtained and used … See more PHA biosynthesis was performed in a 250-mL flask to evaluate PHB production in R. pyridinivorans BSRT1-1 and to select the best carbon and nitrogen … See more A three-variable-five-level design of CCD was used to determine the optimized medium composition for PHB accumulation and the interactive effects of each … See more To enhance the biomass and PHB accumulation of R. pyridinivorans BRST1-1, batch cultivation was carried out in a 10 L stirred-tank bioreactor containing 6 … See more
WebAn extracellular polyhydroxybutyrate depolymerase in Thermus thermophilus HB8. Applied Microbiology and Biotechnology. 2009 Jun;83(4):659-668. doi: 10.1007/s00253-008-1842-2. Powered by Pure, Scopus & Elsevier Fingerprint Engine ... WebIn this study, we constructed a set of single-, double-, and triple-gene deletion strains of Ralstonia eutropha H16 in (p)ppGpp synthase/hydrolase (spoT1), (p)ppGpp synthase (spoT2) and polyhydroxybutyrate (PHB) depolymerase (phaZa1 or phaZa3) genes
WebJun 1, 2009 · The thermophilic bacterium Thermus thermophilus HB8 has been characterized as a polyhydroxybutyrate (PHB)-degrading microorganism since it grows …
WebJun 17, 2016 · Petroleum polymers contribute to non-degradable waste materials and it would therefore be desirable to produce ecofriendly degradable materials. Biodegradation of polyhydroxybutyrate (PHB) in the presence of oligomer hydrolase and PHB depolymerase gave 3-hydroxybutyric acid which could be oxidized to acetyl acetate. Several bacteria and … chuck stalls flood insuranceWebNov 30, 2005 · The crystal structures of polyhydroxybutyrate depolymerase from Penicillium funiculosum and its S39 A mutant complexed with the methyl ester of a trimer substrate of (R)-3-hydroxybutyrate have been determined at resolutions of 1.71 A and 1.66 A, respectively. The enzyme is comprised of a single domain, which represents a circularly … chuck\u0027s towing palm bayWebMaximum production of PHBV depolymerase was observed both at pH 8 and pH 7, 45 °C, 1% substrate concentration and in the presence of lactose as an additional carbon source. Two types of extracellular PHBV depolymerases were purified fromS. kashmirense AF1 by gel permeation chromatography using Sephadex G-75. chuck snow photographyWebTwo methods for accurate poly(3-hydroxybutyrate) (PHB) depolymerase activity determination and quantitative and qualitative hydrolysis product determination are described. The first method is based on online determination of NaOH consumption rates necessary to neutralize 3-hydroxybutyric acid (3HB) and/or 3HB oligomers produced … chuckie\u0027s mom creepypastaWebMany poly-3-hydroxybutyrate (PHB)-degrading enzymes have been studied. But biological roles of 3HB-oligomer hydrolases (3HBOHs) and how PHB depolymerases (PHBDPs) and … chuck rathburn attyWebMar 18, 2009 · The crystal structure of polyhydroxybutyrate depolymerase from Penicillium funiculosum provides insights into the recognition and degradation of biopolyesters. J Mol Biol 2006, 356(4):993–1004. 10.1016/j.jmb.2005.12.028. Article … chuck schumer relation to amyWebMar 1, 2024 · The bacteria produced depolymerase and enzyme activity was maximum after 72 h. The sequencing batch biofilm reactor (SBBR) co-cultured with N. vulgaris SW1 and P. aeruginosa KS10 was operated to remove PHB from the wastewater. Biofilm in the reactor degraded PHB and the production of polyhydroxybutyrate depolymerase influenced on … chuck yeager mig 15